Related Experiment Video
Updated: Feb 14, 2026

Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres
Published on: November 5, 2016
Preparation of Poly(D,L-lactic-co-glycolic acid) Nanoparticles Through Solvent Displacement and Structural Evaluation
Marina Rodrigues Tavares1, Lívia Rodrigues de Menezes1, Michelle Alvares Sarcinelli1
1Instituto de Macromoléculas Professora Eloisa Mano, Universidade Federal do Rio de Janeiro, 21941-970, Rio de Janeiro, Brazil.
Abstract:
The present study obtained coated poly(lactic-co-glycolic acid) nanoparticles using the solvent displacement method aiming to evaluate the effect of the polymer concentration in the pharmaceutical formulations' and polymer properties. Nanosuspensions were evaluated by fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), zeta potential (ZP), thermogravimetric analysis (TGA), X-ray diffraction (XRD) and time-domain nuclear magnetic resonance (TD-NMR). Uniform nanoparticles could be obtained using this method and higher polymer concentrations led to an increase in particle size and negatively charged surfaces were observed. However typical PLGA halo was observed in XRD, diffractograms revealed the Pluronic chains behavior when PLGA concentration changed. Additionally, samples' spin-lattice relaxation times, dipolar interaction and correlation times were evaluated using time-domain nuclear magnetic resonance (TD-NMR), which revealed the interference of PLGA chain packing when Pluronic was incorporated as well as Pluronic chains organization and its behavior around the particle.
Related Concept Videos
Nuclear Magnetic Resonance (NMR): Overview
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
Magnetic Resonance Imaging
Linear Approximation in Time Domain
For a simple pendulum with a mass evenly distributed along its length and the center of mass located at half the pendulum's length,...
Solvents
A...
Resonance and Hybrid Structures
Resonance Structures and Resonance Hybrids
The Lewis structure of a nitrite anion (NO2−) may actually be drawn in two different ways, distinguished by the locations of the N–O and N=O bonds.
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...

