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Updated: Feb 14, 2026

Scalable Syntheses of Graphene Oxide and Reduced Graphene Oxide using Cascade Design Oxidation and Highly Basic Reduction Reactions
Published on: July 3, 2025
Preparation of NH₂-Functionalized Graphene Oxide Nanosheets for Immobilization of NADH Oxidase
1School of Grain Science and Technology, Jiangsu University of Science and Technology, 212009, Zhenjiang, People's Republic of China.
Abstract:
Graphene oxide nanosheets was prepared, modification with 3-aminopropyltriethoxysilane and used as the carrier for immobilization of NADH oxidase (NOX) from Streptococcus mutans. The prepared nanosheets were characterized with FTIR. A high immobilization yield of 92% and activity recovery of 91% were achieved at pH 7.0 and 25 °C. The thermostability of the immobilized NOX was improved about 60% compared with the free enzyme. And the immobilized NOX preserved about 92% of its initial activity after 500 h storage at 4 °C, while free NOX was totally denatured after 200 h at the same conditions. These results indicate that NH2-functionalized graphene oxide nanosheets has a good potential as carrier for immobilization of proteins, especially enzymes.
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