Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

15.0K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
15.0K
Pedigree Analysis01:35

Pedigree Analysis

89.9K
Overview
89.9K
Dimensional Analysis03:40

Dimensional Analysis

65.3K
Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units.
Conversion Factors and Dimensional Analysis
The unit...
65.3K
Load along a Single Axis01:29

Load along a Single Axis

649
In structural engineering, the analysis of beams subjected to varying loads is a critical aspect of understanding the behavior and performance of these structural elements. A common scenario involves a beam subjected to a combination of different load distributions.
Consider a beam of length L subjected to a varying load, which is a combination of parabolic and trapezoidal load distribution along the x-axis. In this case, it is essential to determine the resultant loads, their locations, and...
649
Single Pipe Systems01:24

Single Pipe Systems

467
In pipe flow analysis, problems are typically categorized into three types — Type I, Type II, and Type III — based on the known parameters and the desired outcome. Each type of problem addresses specific engineering requirements using fluid properties, pipe characteristics, and operational conditions.
In a Type I problem, fluid properties (density and viscosity), pipe characteristics (including diameter, length, and surface roughness), and the flow rate or average velocity are...
467
Qualitative Analysis03:46

Qualitative Analysis

25.2K
For solutions containing mixtures of different cations, the identity of each cation can be determined by qualitative analysis. This technique involves a series of selective precipitations with different chemical reagents, each reaction producing a characteristic precipitate for a specific group of cations. Metal ions within a group are further separated by varying the pH, heating the mixture to redissolve a precipitate, or adding other reagents to form complex ions.
For instance, group IV...
25.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Weapons as building blocks: a dual-function microcin in Acinetobacter.

Trends in microbiology·2026
Same author

Bacterial nitrogen-related phosphotransferase systems: new insights into diverse roles in virulence, physiology, and central metabolism.

Journal of bacteriology·2026
Same author

Revisiting a Breathtaking Publication in the History of Molecular Biology.

Journal of molecular biology·2025
Same author

Quorum sensing regulation by the nitrogen phosphotransferase system in <i>Pseudomonas aeruginosa</i>.

Journal of bacteriology·2025
Same author

Quorum sensing regulation by the nitrogen phosphotransferase system in <i>Pseudomonas aeruginosa</i>.

bioRxiv : the preprint server for biology·2025
Same author

Stressosome-independent but RsbT-dependent environmental stress sensing in Bacillus subtilis.

Nature communications·2025

Related Experiment Video

Updated: Feb 14, 2026

Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

12.7K

Single-cell Microfluidic Analysis of Bacillus subtilis.

Matthew T Cabeen1, Richard Losick2

  • 1Department of Microbiology and Molecular Genetics, Oklahoma State University; matthew.cabeen@okstate.edu.

Journal of Visualized Experiments : Jove
|February 15, 2018
PubMed
Summary

Microfluidic technology provides single-cell resolution for long-term bacterial studies. This method enables precise control over growth conditions, revealing authentic gene expression changes in Bacillus subtilis.

More Related Videos

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

21.2K
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

1.7K

Related Experiment Videos

Last Updated: Feb 14, 2026

Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

12.7K
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

21.2K
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

1.7K

Area of Science:

  • Microbiology
  • Biotechnology
  • Cellular Biology

Background:

  • Traditional microbiology methods face limitations in single-cell resolution and environmental control.
  • Microfluidic devices offer uniform growth conditions and long observation times for bacterial studies.
  • Continuous medium flow in microfluidics minimizes environmental variations, allowing for accurate observation of cellular responses.

Purpose of the Study:

  • To demonstrate a microfluidic "mother machine" method for detailed cellular analysis.
  • To showcase the construction, operation, and application of microfluidic devices in microbiology.
  • To illustrate the potential for observing authentic gene expression and growth dynamics in bacteria.

Main Methods:

  • Utilized a "mother machine" microfluidic device for bacterial culture and observation.
  • Demonstrated the construction, plumbing, and cell-loading procedures for the microfluidic system.
  • Implemented a stimulus-response experiment by switching growth media to observe cellular changes.

Main Results:

  • Successfully applied microfluidic technology to Bacillus subtilis for single-cell analysis.
  • Showcased the ability to control growth conditions and expose cells to specific stimuli.
  • Obtained data using a stress-responsive reporter to illustrate the method's capabilities.

Conclusions:

  • Microfluidic technology offers significant advantages over traditional methods for microbiology.
  • The demonstrated "mother machine" method enables precise, high-resolution studies of bacterial behavior.
  • This approach has broad applications for studying bacterial physiology, including sporulation and gene expression.