Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Blood Transfusion01:15

Blood Transfusion

2.5K
Blood transfusion is a critical medical procedure that saves lives and treats various medical conditions. It involves transferring blood from a donor to a recipient. This process requires a thorough understanding of the ABO blood group system and its associated antigens and antibodies.
Blood Transfusion Overview
A blood transfusion is a medical procedure used to replace blood lost due to injury, surgery, or to treat conditions such as anemia or cancer. During a transfusion, donor blood is...
2.5K
Blood Transfusion and Agglutination02:45

Blood Transfusion and Agglutination

15.0K
Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
15.0K
Structure and Function of Platelets01:18

Structure and Function of Platelets

3.8K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
3.8K
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

9.6K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
9.6K
Genetic Screens02:46

Genetic Screens

5.8K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.8K
Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

23.8K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
23.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Current state-of-the-art and gaps in platform trials: 10 things you should know, insights from EU-PEARL.

EClinicalMedicine·2024
Same author

Mobilizing the clinical trial ecosystem to drive adoption of master protocols.

Clinical trials (London, England)·2022
Same author

Comparison of the platelet activation status of single-donor platelets obtained with two different cell separator technologies.

Transfusion·2020
Same author

Screening of red blood cells for extracellular vesicle content as a product quality indicator.

Transfusion·2018
Same author

Microparticle detection to guide platelet management for the reduction of platelet refractoriness in children - A study proposal.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2017
Same author

Could Microparticles Be the Universal Quality Indicator for Platelet Viability and Function?

Journal of blood transfusion·2017

Related Experiment Video

Updated: Feb 14, 2026

Routine Screening Method for Microparticles in Platelet Transfusions
09:49

Routine Screening Method for Microparticles in Platelet Transfusions

Published on: January 31, 2018

16.6K

Routine Screening Method for Microparticles in Platelet Transfusions.

Daniel Millar1, Larry Murphy2, Audrey Labrie1

  • 1Research & Development, LightIntegra Technology Inc.

Journal of Visualized Experiments : Jove
|February 15, 2018
PubMed
Summary

Screening platelet concentrates for microparticle content using dynamic light scattering can differentiate activated from non-activated platelets. This quality improvement initiative helps optimize the use of scarce platelet transfusions for better patient care.

More Related Videos

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

14.4K
Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
10:25

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Published on: March 19, 2016

10.0K

Related Experiment Videos

Last Updated: Feb 14, 2026

Routine Screening Method for Microparticles in Platelet Transfusions
09:49

Routine Screening Method for Microparticles in Platelet Transfusions

Published on: January 31, 2018

16.6K
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

14.4K
Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
10:25

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Published on: March 19, 2016

10.0K

Area of Science:

  • Hematology
  • Biotechnology
  • Quality Improvement in Transfusion Medicine

Background:

  • Platelet concentrates can contain microparticles (MP) from stressed or activated platelets.
  • Platelets are crucial for coagulation, hemostasis, and innate immunity, generating MPs during activation.
  • Current platelet inventory management lacks methods to differentiate activated from non-activated platelets.

Purpose of the Study:

  • To introduce a screening method for routine platelet inventory management in hospital blood banks.
  • To differentiate activated (high MP) from non-activated (low MP) platelets in transfusion units.
  • To optimize the utilization of the scarce blood product, platelets.

Main Methods:

  • Utilizing dynamic light scattering (DLS) for microparticle detection.
  • Implementing a protocol for sampling platelet concentrates.
  • Analyzing microparticle content to identify platelet activation status.

Main Results:

  • The DLS-based method enables differentiation of activated and non-activated platelets.
  • Screening allows for the identification of platelet concentrates with high microparticle content.
  • This facilitates the selection of non-activated platelets for specific patient populations.

Conclusions:

  • Microparticle screening offers a novel approach to platelet quality improvement.
  • Differentiating platelet activation status can enhance transfusion strategies.
  • Optimized platelet inventory management may improve outcomes for hematology-oncology patients.