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Related Experiment Video

Updated: Feb 14, 2026

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Published on: February 1, 2018

Detecting Anastasis In Vivo by CaspaseTracker Biosensor.

Ho Man Tang¹, Ming Chiu Fung², Ho Lam Tang³

¹Institute for Basic Biomedical Sciences, Johns Hopkins University School of Medicine; School of Life Sciences, Chinese University of Hong Kong; homantang@jhmi.edu.

Journal of Visualized Experiments : Jove

|February 15, 2018

Summary

Scientists developed a new biosensor system to track cell recovery after death. This tool, CaspaseTracker, permanently labels cells that have reversed late-stage cell death, aiding research into anastasis and its therapeutic potential.

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Last Updated: Feb 14, 2026

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

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Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Published on: February 16, 2015

Area of Science:

Cell Biology
Developmental Biology
Biotechnology

Background:

Anastasis is a cell recovery phenomenon where dying cells reverse late-stage cell death.
Promoting anastasis could aid tissue repair (e.g., cardiomyocytes, neurons), while suppressing it may enhance cancer therapy efficacy.
Tracking anastatic cells in vivo has been challenging due to their normal appearance post-recovery.

Purpose of the Study:

To develop and present in vivo protocols for a novel dual biosensor system, CaspaseTracker.
To enable the identification and permanent tracking of anastatic cells in live organisms.
To facilitate future research on the mechanisms and implications of cell recovery.

Main Methods:

Development of Drosophila and mammalian CaspaseTracker biosensor systems.
In vivo protocols for generating and using the CaspaseTracker dual biosensor in Drosophila melanogaster.
Utilizing the biosensor to label cells recovering from caspase activation, a hallmark of apoptosis.

Main Results:

The CaspaseTracker system can permanently label cells that have recovered from late-stage apoptosis.
It simultaneously identifies active apoptotic processes and tracks recovery from other caspase-involved cell death pathways.
The protocol allows continuous monitoring of anastatic cells and their progeny in vivo.

Conclusions:

The CaspaseTracker biosensor provides a crucial tool for studying anastasis in live animals.
This system overcomes previous limitations in tracking cell recovery after death.
Enables deeper investigation into the biological functions, mechanisms, and therapeutic applications of anastasis.