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The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis
Published on: January 29, 2018
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The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis
Mathew Tata1, Christiana Ruhrberg2
1UCL Institute of Ophthalmology.
Journal of Visualized Experiments : Jove
|February 15, 2018
Summary
The mouse hindbrain offers a better model for studying mammalian neurogenesis than the forebrain. This study presents a simple method to analyze neural progenitor cell behavior in wholemount hindbrain preparations.
Area of Science:
- Developmental biology
- Neuroscience
- Genetics
Background:
- The mouse embryo forebrain is a common model for mammalian neurogenesis.
- Its folded structure limits wholemount analysis of neurogenesis patterns.
- Forebrain-specific progenitor subtypes may limit the generalizability of findings.
Purpose of the Study:
- To introduce the mouse hindbrain as a more accessible model for studying mammalian neurogenesis.
- To present a simple and quick method for analyzing neural progenitor cell behavior in the mouse hindbrain.
Main Methods:
- Utilizing wholemount preparations of the mouse embryo hindbrain.
- Employing tissue sections for detailed observation.
- Leveraging existing cell lineage reporter and mutant mouse strains.
Main Results:
- The mouse hindbrain is amenable to wholemount analysis for observing neurogenesis.
- Tissue sections allow for detailed spatiotemporal analysis of neural progenitors.
- The hindbrain model facilitates downstream applications like cell isolation and molecular analysis.
Conclusions:
- The mouse hindbrain is a powerful and versatile model for studying developmental neurogenesis.
- The presented method enables efficient analysis of neural progenitor cell behavior in the hindbrain.
- This approach enhances the study of mammalian neurogenesis mechanisms.
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