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Related Experiment Video

Updated: Feb 14, 2026

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Percutaneous Contrast Echocardiography-guided Intramyocardial Injection and Cell Delivery in a Large Preclinical

Alejandro Giraldo1, Jesús Talavera López2, Maria Josefa Fernandez-Del-Palacio3

  • 1Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading; a.giraldoramirez@reading.ac.uk.

Journal of Visualized Experiments : Jove
|February 15, 2018
PubMed
Summary

A novel minimally invasive technique for intramyocardial injection in rabbits enables precise delivery of cardiac regenerative therapies. This method is safe, effective, and crucial for preclinical testing of cell and gene therapies for heart failure with reduced ejection fraction.

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Area of Science:

  • Regenerative Medicine
  • Cardiovascular Research
  • Preclinical Animal Models

Background:

  • Cell and gene therapies show promise for cardiac regeneration in heart failure with reduced ejection fraction (HFrEF).
  • Extensive preclinical studies in large animal models are essential to validate safety and efficacy before human application.
  • Small rodent models have limitations in translating findings to clinical settings, necessitating the use of larger, more relevant models.

Purpose of the Study:

  • To demonstrate a minimally invasive, percutaneous contrast echocardiography-guided intramyocardial injection (IMI) technique in a rabbit model.
  • To establish a safe, effective, and reproducible method for targeted delivery of injectates, including cells, into the myocardium.
  • To facilitate hypothesis testing for cardiac regenerative therapeutics using a cost-effective and clinically relevant large animal model.

Main Methods:

  • Utilized a percutaneous contrast echocardiography-guided intramyocardial injection (IMI) technique.
  • Employed a widely available clinical echocardiography system for guidance.
  • The procedure was optimized for targeted delivery into multiple myocardial locations in rabbits.

Main Results:

  • The demonstrated IMI technique is minimally invasive, safe, and well-tolerated in rabbits.
  • The technique allows for highly effective and targeted delivery of injectates into the myocardium.
  • Competency in performing the procedure can be achieved by researchers with basic ultrasound knowledge, with the entire process taking approximately 25 minutes.

Conclusions:

  • Percutaneous contrast echocardiography-guided IMI is a versatile and effective method for preclinical cardiac regeneration studies in rabbits.
  • This technique supports the routine use of rabbit models for evaluating the potential of novel cardiac regenerative therapeutics.
  • The established protocol provides a standardized approach for advancing cell and gene therapies for HFrEF towards clinical translation.