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Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
Published on: February 21, 2019
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A Versatile and Robust Approach to Stimuli-Responsive Protein Multilayers with Biologically Enabled Unique Functions.
Xue-Jian Zhang1,2, Xiao-Wei Wang1, Xiao-Di Da1
1Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering , Peking University , Beijing 100871 , People's Republic of China.
Biomacromolecules
|February 15, 2018
Summary
Researchers developed a novel protein layer-by-layer assembly method using "Tag-Catcher" reactions. This technique creates robust, entirely protein-based materials with enhanced uranyl sequestration and recycling capabilities.
Area of Science:
- Materials Science
- Biotechnology
- Chemical Engineering
Background:
- Protein-based materials offer unique functionalities but require advanced processing for integration.
- Current methods often struggle to combine protein properties with other materials effectively.
Purpose of the Study:
- To develop a facile and robust covalent layer-by-layer assembly method for entirely protein-based multilayers.
- To demonstrate the enhanced uranyl sequestration and recycling capabilities of these protein-based materials.
Main Methods:
- Utilized orthogonal
- Tag-Catcher
- reactions for covalent assembly of native telechelic proteins.
- Immobilized super uranyl-binding protein (SUP) on silica gel to create protein multilayers.
Main Results:
- Achieved tunable capacity and enhanced uranyl sequestration using the protein multilayers.
- Demonstrated high recovery rates (∼90% in water, ∼60% in synthetic seawater) over 10+ cycles, indicating material resilience.
- Created the first entirely protein-based multilayers via covalent layer-by-layer assembly.
Conclusions:
- The developed method provides a versatile platform for protein immobilization, enhancing both function and resilience.
- This approach expands the capabilities of genetically encoded protein-based materials for applications like environmental remediation.
- The protein multilayers exhibit stimuli-sensitive behaviors and properties superior to synthetic alternatives.
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