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    We developed a low-power, high-resolution multiphoton microscopy technique using a nitrogen vacancy (NV) center in diamond. This method significantly enhances resolution for biological imaging and quantum sensing applications.

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    Area of Science:

    • Optics and Photonics
    • Quantum Technologies
    • Biomedical Imaging

    Background:

    • Multiphoton microscopy (MPM) is a key technique for 3D biological imaging.
    • Nitrogen vacancy (NV) centers in diamond offer unique optical properties for sensing and imaging.
    • Improving the resolution of MPM is crucial for detailed biological studies.

    Purpose of the Study:

    • To demonstrate a novel low-power, high-resolution MPM technique.
    • To utilize the photon-induced charge state conversion process in NV centers.
    • To enhance resolution beyond conventional microscopy methods.

    Main Methods:

    • Employed a nitrogen vacancy (NV) center in diamond as the imaging probe.
    • Utilized continuous wave green and orange lasers for pumping and detection.
    • Leveraged two-photon charge state conversion for signal generation.
    • Operated with a low excitation laser power of 40 μW.

    Main Results:

    • Achieved significantly improved axial and lateral resolution.
    • Demonstrated a resolution enhancement of approximately 1.5 times compared to confocal microscopy.
    • Validated the low-power requirements for high-resolution imaging.

    Conclusions:

    • The developed MPM technique offers superior resolution for biological imaging.
    • This method holds potential for advancing NV center-based quantum sensing.
    • Provides a pathway for higher-fidelity nanoscale imaging and sensing.