Related Experiment Video For Antibiotic resistance
Updated: Feb 14, 2026

Author Spotlight: Detection and Treatment of Helicobacter pylori Infection
Published on: July 28, 2023
Simultaneous detection of human CYP2C19 polymorphisms and antibiotic resistance of Helicobacter pylori using a
Jun Zhang1, Jing Zhong2, Jian Ding1
1Department of Gastroenterology, Huzhou Central Hospital, Huzhou, Zhejiang Province, China.
Objectives:
A personalised diagnosis kit for Helicobacter pylori that employs visual gene chip technology for the simultaneous detection of CYP2C19 polymorphisms and clarithromycin/levofloxacin antibiotic resistance was evaluated.
Methods:
Gastric antrum mucosa biopsy specimens of 394 patients were tested using the kit. DNA sequencing and antibiotic susceptibility testing of the H. pylori were also performed.
Results:
In total, 267 (67.8%) of the 394 specimens were positive for H. pylori using the kit and DNA sequencing, and 136 (34.5%) were positive by culturing. For human CYP2C19 and the bacterial 23S rRNA and gyrA genes, the concordance rates were 92.4% (364/394), 96.6% (258/267) and 97.0% (259/267) between the kit and DNA sequencing results, respectively. For clarithromycin and levofloxacin resistance, the concordance rates were 90.4% (123/136) and 81.6% (111/136) between the kit and antibiotic susceptibility testing results.
Conclusions:
The personalised diagnosis kit for H. pylori provides useful information for the choice of proton pump inhibitor and antibiotic in combination therapy.
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