Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

700
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
700
What are Populations and Communities?00:30

What are Populations and Communities?

38.0K
Overview
38.0K
Methods for Controlling Microbial Growth01:29

Methods for Controlling Microbial Growth

1.8K
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
1.8K
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

915
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
915
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

1.6K
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
1.6K
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

1.9K
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shenfu Decoction Extends Survival Time of Seawater-Induced Hypothermia in Rats: The Role of Metabolomics and Gut Microbiota.

Current drug metabolism·2026
Same author

Ultrasound-Guided Radiofrequency Ablation of Benign Thyroid Nodules: 2-Year Follow-Up Results from a Single Center.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine·2026
Same author

High-Resolution Spatiotemporal Characterization of PM<sub>2.5</sub> across On-Road Microenvironments Using Large-Scale Taxi-Based Mobile Monitoring in a Chinese City.

Environmental science & technology·2026
Same author

An Ecological Impact Assessment Framework and Integrated Management Pathway for Large-Scale Chemical Mosquito Control in Urban Areas.

ACS environmental Au·2026
Same author

Lipase Immobilized in Hollow Mesoporous Carbon Spheres: Enzymatic Properties and Applications in the Production of Geranyl Acetate.

Journal of agricultural and food chemistry·2026
Same author

Multistability of quantum entanglement and squeezing in nonlinear cavity magnonic systems.

Optics express·2026
Same journal

The response of microbial necromass C and its contribution to SOC to vinasse biochar based on a pot experiment.

Frontiers in microbiology·2026
Same journal

Rhodamine B/gold nanoparticles-coloaded UiO-66 as a novel probe for highly sensitive and dual-mode detection of Potato virus S in field samples.

Frontiers in microbiology·2026
Same journal

Adaptive reprogramming of carbon-nitrogen metabolism in <i>Klebsiella aerogenes</i> under nitrate-rich conditions.

Frontiers in microbiology·2026
Same journal

Predatory <i>Myxococcus fulvus</i> strain KS01 confers biocontrol and growth promotion in cotton through multifaceted antifungal mechanisms.

Frontiers in microbiology·2026
Same journal

Understanding the influence of sward type and dairy cow breed on enteric methane emissions through investigation of the rumen microbiome.

Frontiers in microbiology·2026
Same journal

Morpho-cultural, pathogenic, and molecular diversity, and population structure analysis of <i>Bipolaris sorokiniana</i> causing spot blotch disease in wheat.

Frontiers in microbiology·2026
See all related articles

Related Experiment Video

Updated: Feb 14, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
08:13

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

17.9K

Microbial Communities and Diversities in Mudflat Sediments Analyzed Using a Modified Metatranscriptomic Method.

Yong-Wei Yan1, Qiu-Yue Jiang1, Jian-Gong Wang1

  • 1Key Laboratory for Biodiversity Science and Ecological Engineering, Ministry of Education, Institute of Biodiversity Science, School of Life Sciences, Fudan University, Shanghai, China.

Frontiers in Microbiology
|February 16, 2018
PubMed
Summary
This summary is machine-generated.

This study reveals higher microbial diversity in Dongtan mudflats using metatranscriptomics. Total nitrogen and carbon content significantly influence microbial distribution, with PCR methods underestimating microbial activity.

Keywords:
SSU rRNAbiascommunity compositionintertidal mudflatmetatranscriptome

More Related Videos

Biology of Microbial Communities - Interview
14:42

Biology of Microbial Communities - Interview

Published on: May 28, 2007

9.1K
Investigating the Microbial Community in the Termite Hindgut - Interview
21:02

Investigating the Microbial Community in the Termite Hindgut - Interview

Published on: May 28, 2007

11.2K

Related Experiment Videos

Last Updated: Feb 14, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
08:13

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

17.9K
Biology of Microbial Communities - Interview
14:42

Biology of Microbial Communities - Interview

Published on: May 28, 2007

9.1K
Investigating the Microbial Community in the Termite Hindgut - Interview
21:02

Investigating the Microbial Community in the Termite Hindgut - Interview

Published on: May 28, 2007

11.2K

Area of Science:

  • Microbial ecology
  • Environmental science
  • Biogeochemistry

Background:

  • Intertidal mudflats are crucial for global nutrient cycles but their microbial communities are poorly understood.
  • Dongtan wetland, a unique ecosystem, requires in-depth microbial investigation.
  • Understanding microbial activity is key to comprehending nutrient cycling in these dynamic environments.

Purpose of the Study:

  • To investigate the depth-wise distribution of potentially active microbial communities in Dongtan mudflat sediments.
  • To compare microbial diversity and composition using metatranscriptomics versus traditional 16S rRNA gene amplicon sequencing.
  • To identify environmental factors influencing microbial community structure and activity.

Main Methods:

  • Modified metatranscriptomic sequencing of small subunit ribosomal RNA (SSU rRNA) from sediment samples.
  • Analysis of prokaryotic diversity and community composition.
  • Measurement and statistical analysis of environmental factors (total nitrogen, carbon content) in relation to microbial profiles.

Main Results:

  • Metatranscriptomics revealed 2-3 times higher diversity indices for potentially active microbes compared to 16S rRNA gene amplicon data.
  • Bacteria, particularly Chloroflexi, Acidobacteria, Bacteroidetes, and Proteobacteria, were dominant. Archaea included Bathyarchaeota and Thermoplasmatales.
  • Total nitrogen and carbon content significantly correlated with the depth-wise distribution of bacterial and archaeal communities.
  • PCR-based methods, especially at the DNA level, significantly underestimated the activity of key taxa like Desulfobacterales and Anaerolineaceae.

Conclusions:

  • Metatranscriptomics provides a more comprehensive view of active microbial communities and their diversity in intertidal mudflats.
  • Sediment geochemistry, specifically nitrogen and carbon content, plays a critical role in structuring microbial communities at different depths.
  • The study highlights the limitations of DNA-based methods for assessing microbial activity and emphasizes the value of RNA-based approaches for ecological studies.