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Updated: Feb 14, 2026

Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
Published on: November 6, 2021
Management of Multiple Gingival Recessions with the VISTA Technique: An 18-Month Clinical Case Series
Abstract:
The purpose of the present study was to evaluate the efficacy of the vestibular incision subperiosteal tunnel access (VISTA) technique with collagen membrane, bioactive glass, and platelet-rich fibrin. A total of 22 systemically and periodontally healthy subjects with multiple gingival recession defects (Miller Class I and II) in the maxillary esthetic zone were enrolled in the study. The primary outcome measures were mean root coverage and alteration in gingival thickness. There were no surgical complications; however, one patient reported swelling of the upper lip area on the first postoperative day, which resolved spontaneously. A statistically significant increase in gingival thickness (0.45 mm) and 94.17% ± 8.42% mean root coverage was observed at 18 months. Within the limitations of this study, it was concluded that the VISTA technique augmented with collagen membrane, bioactive glass, and platelet-rich fibrin is a predictable modality in multiple recession management.
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