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Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Analysis of the Complete Genome Sequence of Cucumber mosaic virus Strain K
Richard Moyle1, Lara-Simone Pretorius1, Louise S Shuey1
1Nexgen Plants Pty. Ltd., School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Queensland, Australia.
Abstract:
The complete genome sequence of Cucumber mosaic virus strain K was determined by deep RNA sequencing. The tripartite genome consists of a 3,382-nucleotide (nt) RNA1, a 3,050-nt RNA2, and a 2,218-nt RNA3 segment. Phylogenetic analysis placed RNA1 and RNA2 in subgroup IB. However, RNA3 grouped with subgroup IA isolates, indicating a likely recombination event.
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