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Published on: March 27, 2015
Directional preparation of anticoagulant-active sulfated polysaccharides from Enteromorpha prolifera using artificial
Jiefen Cui1, Yinping Li1,2, Shixin Wang3
1College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, PR China.
Abstract:
The sulfated polysaccharides from Enteromorpha prolifera (PE) are a potential source of anticoagulant agents. In this study, the PE was degraded by specific degradase and five hydrolysis products with different molecular weights were prepared. The product of 206 kDa is a kind of high rhamnose-containing polysaccharide with sulfate ester (34.29%). It could effectively prolong the activated partial thromboplastin time (APTT), which indicated inhibition of the intrinsic coagulation pathway. The artificial neural network (ANN) was built to realize the directional preparation of anticoagulant-active polysaccharides. Based on monitoring glucose concentration on-line, a visualization system of enzymatic hydrolysis was developed to simplify the operation of ANN. The model could be further applied to predict molecular weights of polysaccharides that possess diverse biological activities.
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