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Updated: Feb 14, 2026

Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites
Published on: January 3, 2011
Protection from experimental cerebral malaria with a single intravenous or subcutaneous whole-parasite immunization
Kirsten Heiss1,2, Marion Irmgard Maier1,3, Angelika Hoffmann4,5
1Centre for Infectious Diseases, Parasitology Unit, Heidelberg University Hospital, Heidelberg, Germany.
Abstract:
Cerebral malaria is a life-threatening complication of Plasmodia infection and a major cause of child mortality in Sub-Saharan Africa. We report that protection from experimental cerebral malaria in the rodent model is obtained by a single intravenous or subcutaneous whole-parasite immunization. Whole-parasite immunization with radiation-attenuated sporozoites was equally protective as immunization with non-attenuated sporozoites under chemoprophylaxis. Both immunization regimens delayed the development of blood-stage parasites, but differences in cellular and humoral immune mechanisms were observed. Single-dose whole-parasite vaccination might serve as a relatively simple and feasible immunization approach to prevent life-threatening cerebral malaria.
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