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Peptidoglycan Recognition Protein 3 Does Not Alter the Outcome of Pneumococcal Pneumonia in Mice

Anshu Shrivastav1, Alexander N Dabrowski1, Claudia Conrad1

  • 1Department of Internal Medicine/Infectious Diseases and Pulmonary Medicine, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Frontiers in Microbiology
|February 17, 2018
PubMed

Insights

Pneumolysin, a protein from Streptococcus pneumoniae, does not significantly impact pneumonia outcomes. Studies show PGLYRP3 (PGRP-LL) is not essential for antibacterial defense in pneumococcal pneumonia models.

Area of Science:

  • Immunology
  • Microbiology
  • Pulmonology

Background:

  • Community-acquired pneumonia (CAP) caused by pneumococci has high mortality rates.
  • Antibiotic resistance necessitates exploring endogenous antimicrobial peptides as novel therapeutic targets.
  • PGLYRP3 (PGRP-LL) is an endogenous antimicrobial protein with potential roles in pneumonia.

Purpose of the Study:

  • To analyze the expression pattern of PGLYRP3 in lung cells during pneumococcal pneumonia.
  • To investigate the functional role of PGLYRP3 in combating Streptococcus pneumoniae infection using a murine model.

Main Methods:

  • Expression analysis of PGLYRP3 in alveolar epithelial cells, macrophages, and neutrophils.
  • Utilizing PGLYRP3 knockout (PGLYRP3KO) mice to assess the protein's function in a murine pneumococcal pneumonia model.
  • Evaluating bacterial load, cytokine response, immune cell composition, histopathology, clinical symptoms, and survival rates.

Main Results:

  • PGLYRP3 expression was detected in all studied lung cell types.
  • Pneumococcus-induced PGLYRP3 expression was observed specifically in neutrophils and alveolar macrophages.
  • No significant differences were found between wild-type and PGLYRP3KO mice in bacterial load, immune response, or clinical outcomes.

Conclusions:

  • PGLYRP3 is expressed in key lung immune cells during pneumococcal pneumonia.
  • PGLYRP3 does not appear to play a critical role in the host's defense against pneumococcal pneumonia.
  • PGLYRP3 is dispensable for antibacterial defense in this murine pneumonia model.
Keywords:
PGLYRPPGRPStreptococcus pneumoniaeinnate immunitypeptidoglycan recognition proteinspneumococcal pneumonia

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