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Updated: Feb 14, 2026

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
Published on: September 28, 2022
Identification and expression profiles of novel odorant binding proteins and functional analysis of OBP99a in
Jing Zhang1, Deye Luo1, Peng Wu1
1State Key Laboratory of Agricultural Microbiology, Key Laboratory of Horticultural Plant Biology, Ministry of Education and Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Abstract:
Odorant-binding proteins (OBPs) in insects are essential for mating and oviposition host selection. How these OBPs respond to different hosts at the mRNA level and their effects on behavior remain poorly characterized. The oriental fruit fly Bactrocera dorsalis is a highly invasive agricultural pest with an extremely broad host range and high fecundity. Based on our previously constructed B. dorsalis transcriptome, six OBPs that were differentially expressed during three different physiological adult stages were identified. A phylogenetic tree was constructed to illustrate the relationships of these six OBPs with OBP sequences from three other dipteran species (Drosophila melanogaster, Anopheles gambiae, and Ceratitis capitata). The spatiotemporal expression profiles of the six OBPs were analyzed using quantitative real-time PCR. Our results revealed that OBP19c, OBP44a, OBP99a, and OBP99d were abundantly expressed from the prepupa stage to the adult stage, and most of the OBPs were mainly expressed in the head, wings, and antennae. The expression levels of these OBPs were upregulated when female flies were exposed to their preferred hosts. Silencing OBP99a resulted fewer eggs being laid compared with the control group when the females were exposed to their preferred host, that is, banana, whereas more eggs were laid when a non-preferred host, that is, tomato, was used. Furthermore, silencing OBP99a led to sexually dimorphic mating behavior. dsOBP99a-injected males dramatically reduced courtship, whereas enhanced courtship was observed in the treated females. These data indicate that OBPs may participate in different biological processes of B. dorsalis. Our study will provide insight into the molecular mechanism of chemoreception and help develop ecologically friendly pest-control strategies.
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