Novel automated immune turbidimetric assay for routine urinary cystatin-C determinations
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Published on: November 10, 2016
Balázs Szirmay1, Péter Kustán1,2, Zoltán Horváth-Szalai1
1Department of Laboratory Medicine, University of Pécs Medical School, 7624 Pécs, Ifjúság u. 13, Hungary.
Aim:
There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders.
Materials & Methods:
A particle-enhanced immune turbidimetric assay was adapted and validated on a Cobas 8000/c502 analyzer. Urine samples of different patient groups were also analyzed.
Results:
Our method showed excellent analytical performance. U-CYSC/u-creatinine (u-CREAT) was higher in sepsis-related acute kidney injury group (p < 0.001) compared with controls and to patients with chronic hypertension and Type 2 diabetes.
Conclusion:
We validated a fast, sensitive, fully automated u-CYSC assay which is ideal for routine use and might be a potential complementary laboratory test to evaluate renal tubular function.
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