Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

9.3K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
9.3K
Imaging Studies IV: Magnetic Resonance Imaging01:27

Imaging Studies IV: Magnetic Resonance Imaging

296
Introduction:Magnetic Resonance Imaging, or MRI, can include a specialized imaging technique of the urinary system known as Magnetic Resonance Urography (MRU). This radiation-free technique uses strong magnetic fields and radio waves to produce detailed images with the help of a computer. MRU is particularly effective for visualizing fluid-filled structures like the kidneys, ureters, and bladder.Applications of MRI in the Genitourinary SystemKidneys and Ureters: MRI detects tumors, cysts,...
296
Imaging Studies for Cardiovascular System IV: CMRI01:21

Imaging Studies for Cardiovascular System IV: CMRI

420
Cardiovascular magnetic resonance imaging, or CMRI, is a non-invasive diagnostic test that employs a magnetic field and radiofrequency waves to create precise images of the heart and arteries. It provides comprehensive information about cardiac anatomy, function, perfusion, and tissue characterization without ionizing radiation.IndicationsCMRI diagnoses various heart conditions, including tissue damage from heart attacks, ischemic heart disease, myocarditis, aortic issues (tears, aneurysms,...
420
Protein Complex Assembly02:41

Protein Complex Assembly

16.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.9K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

3.0K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
3.0K
Complex Numbers01:29

Complex Numbers

344
The real number system cannot represent the square root of a negative number, which restricts solutions for certain equations, such as quadratics with negative discriminants. To address this, the complex number system was developed, introducing the imaginary unit i, where i = √(-1). This extension allows for the representation of all roots, including those involving negative radicands.A complex number is written in the form x + yi, where x and y are real numbers. Here, x represents the...
344

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Controlling Amyloid Assembly Dynamics Using Spin Interfaces.

ACS nano·2025
Same author

Tailoring Peptide Coacervates for Advanced Biotechnological Applications: Enhancing Control, Encapsulation, and Antioxidant Properties.

ACS applied materials & interfaces·2025
Same author

EspH utilizes phosphoinositide and Rab binding domains to interact with plasma membrane infection sites and Rab GTPases.

Gut microbes·2024
Same author

Self-Assembly of a Dipeptide with a Reduced Amount of Copper into Antifungal and Antibacterial Particles.

Biomacromolecules·2024
Same author

Topology and function of translocated EspZ.

mBio·2023
Same author

Titanium complexes affect <i>Bacillus subtilis</i> biofilm formation.

RSC medicinal chemistry·2023

Related Experiment Video

Updated: Feb 14, 2026

Imaging Cell Viability on Non-transparent Scaffolds &#8212; Using the Example of a Novel Knitted Titanium Implant
07:28

Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant

Published on: September 7, 2016

11.9K

Fluorescent antitumor titanium(iv) salen complexes for cell imaging.

Avia Tzubery1, Naomi Melamed-Book, Edit Y Tshuva

  • 1Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel. edit.tshuva@mail.huji.ac.il.

Dalton Transactions (Cambridge, England : 2003)
|February 17, 2018
PubMed
Summary

Two novel titanium complexes were developed for cancer research. One showed significant cancer cell toxicity by accumulating near the nucleus and causing phototoxicity, while the other was inactive.

More Related Videos

Digital Printing of Titanium Dioxide for Dye Sensitized Solar Cells
08:19

Digital Printing of Titanium Dioxide for Dye Sensitized Solar Cells

Published on: May 4, 2016

13.2K
Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
07:42

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Published on: April 2, 2008

13.9K

Related Experiment Videos

Last Updated: Feb 14, 2026

Imaging Cell Viability on Non-transparent Scaffolds &#8212; Using the Example of a Novel Knitted Titanium Implant
07:28

Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant

Published on: September 7, 2016

11.9K
Digital Printing of Titanium Dioxide for Dye Sensitized Solar Cells
08:19

Digital Printing of Titanium Dioxide for Dye Sensitized Solar Cells

Published on: May 4, 2016

13.2K
Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
07:42

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Published on: April 2, 2008

13.9K

Area of Science:

  • Inorganic Chemistry
  • Materials Science
  • Biomedical Research

Background:

  • Titanium(IV) complexes with salen ligands are explored for potential applications.
  • Fluorescent probes are valuable tools for live-cell imaging and mechanistic studies.
  • Understanding cellular uptake and localization is crucial for developing effective therapeutic agents.

Purpose of the Study:

  • To synthesize and characterize two distinct fluorescent salen titanium(IV) complexes.
  • To evaluate the cytotoxicity of these complexes against human cancer cells.
  • To investigate the cellular uptake, localization, and phototoxicity mechanisms of the complexes.

Main Methods:

  • Synthesis and characterization of two differently substituted fluorescent salen Ti(iv) complexes.
  • In vitro cytotoxicity assays on human cancer cell lines.
  • Live-cell imaging using fluorescence microscopy to track complex localization.
  • Phototoxicity studies under continuous irradiation.

Main Results:

  • One complex exhibited high cytotoxicity against human cancer cells, while the other was inactive.
  • Both complexes successfully penetrated cancer cells but were not found in the nucleus.
  • The inactive complex was sequestered in endocytic vesicles.
  • The active complex localized to the perinuclear region and induced phototoxicity upon irradiation.

Conclusions:

  • Differential cellular localization dictates the cytotoxic activity of salen Ti(iv) complexes.
  • The active complex shows potential as a photodynamic therapeutic agent for cancer.
  • Further investigation into the perinuclear accumulation and photodynamic effects is warranted.