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Updated: Feb 14, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.4K

Expression and therapeutic implications of cyclin-dependent kinase 4 (CDK4) in osteosarcoma

Yubing Zhou1, Jacson K Shen2, Zujiang Yu3

  • 1Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, People's Republic of China; Sarcoma Biology Laboratory, UCLA Orthopaedic Surgery, Los Angeles, CA 90095, USA.

Biochimica Et Biophysica Acta. Molecular Basis of Disease
|February 17, 2018
PubMed

Insights

Cyclin-dependent kinase 4 (CDK4) is highly expressed in osteosarcoma and linked to metastasis. Inhibiting CDK4 with palbociclib or siRNA reduces tumor growth, migration, and induces apoptosis, suggesting CDK4 as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Cyclin-dependent kinase 4 (CDK4) is implicated in various human cancers.
  • CDK4 inhibitors like palbociclib are FDA-approved for breast cancer.
  • The role of CDK4 in osteosarcoma remains largely uncharacterized.

Purpose of the Study:

  • To investigate the expression of CDK4 in osteosarcoma.
  • To evaluate the therapeutic potential of CDK4 inhibition in osteosarcoma treatment.

Main Methods:

  • Immunohistochemical analysis of osteosarcoma tissue microarrays.
  • In vitro studies using palbociclib and small interfering RNA (siRNA) for CDK4 inhibition.
  • Cell proliferation, cell cycle, apoptosis, and migration assays (flow cytometry, wound healing).

Main Results:

  • CDK4 was significantly overexpressed in osteosarcoma tissues and cell lines compared to normal osteoblasts.
  • Elevated CDK4 expression correlated with increased metastasis and poorer prognosis.
  • CDK4 inhibition suppressed osteosarcoma cell proliferation, growth, and migration.
  • CDK4 knockdown led to G1 cell cycle arrest and induced apoptosis, impacting the CDK4/6-cyclinD-Rb pathway.

Conclusions:

  • CDK4 is a promising therapeutic target for osteosarcoma.
  • Targeting CDK4 may offer a novel treatment strategy for osteosarcoma patients.
Keywords:
Cell cycleCyclin-dependent kinase 4OsteosarcomaPalbociclibRb

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