Potential value of circulating microRNA-126 and microRNA-210 as biomarkers for type 2 diabetes with coronary artery...

Macrovascular complications are the main cause of morbidity and mortality among the diabetic patients. MicroRNAs (miRNAs), a family of small non-coding RNAs, play vital roles in the regulation of blood glucose level and the concurrent cardiovascular complications of type 2 diabetes. We hypothesized that plasma miR-126 and miR-210 are linked to coronary artery disease (CAD) in these diabetes patients.

Fasting blood samples were collected from 20 healthy volunteers and 100 patients with diabetes (54 patients without CAD and 46 patients with CAD). Plasma miR-126 and miR-210 expressions were assessed by quantitative real time PCR. Specificity and sensitivity of miR-126 and miR-210 to discriminate CAD with diabetes was determined by receiver operating characteristic curve analysis. Correlations between miR-126 and miR-210 and studied characteristics in diabetes patients with and without CAD were compared.

Plasma relative expressions of miR-126 and miR-210 were 0.38 ± 0.03 and 5.3 ± 0.56 in diabetes alone vs. 0.08 ± 0.03 and 21.44 ± 0.97 in diabetes with CAD, respectively (both p < 0.0001). Levels of miR-126 and miR-210 significantly correlated with certain glycemic and lipid indices. The miRNAs significantly discriminated between diabetes with and without CAD at cut-off values of 0.055 (sensitivity 91.3%, specificity 100%) for miR-126 and of 17.59 (sensitivity 93.5%, specificity 100%) for miR-210.

Plasma miR-126 and miR-210 levels may be biomarkers for diabetes with or without CAD.