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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

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The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
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Tumor Progression02:07

Tumor Progression

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Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
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What is Gene Expression?01:42

What is Gene Expression?

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
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In ovo Expression of MicroRNA in Ventral Chick Midbrain
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In ovo Expression of MicroRNA in Ventral Chick Midbrain

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MicroRNA-411 promoted the osteosarcoma progression by suppressing MTSS1 expression.

Ning Xu1, Weiliang Yang1, Yang Liu1

  • 1Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng St, Nangang, Harbin, Heilongjiang, China.

Environmental Science and Pollution Research International
|February 18, 2018
PubMed
Summary
This summary is machine-generated.

MicroRNA 411 (miR-411) is elevated in osteosarcoma patients and promotes tumor growth and migration. It functions as an oncogene by suppressing metastasis suppressor protein 1 (MTSS1) expression.

Keywords:
MTSS1MicroRNAsOncogeneOsteosarcomamiR-411

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are critical regulators in various cancers.
  • Osteosarcoma is a primary bone malignancy with complex molecular underpinnings.

Purpose of the Study:

  • To investigate the expression and functional role of miR-411 in human osteosarcoma.
  • To identify the downstream targets and molecular mechanisms of miR-411 in osteosarcoma.

Main Methods:

  • Quantitative real-time PCR to assess miR-411 and MTSS1 expression in patient serum, tissues, and cell lines.
  • Cell proliferation and migration assays to evaluate the functional impact of miR-411.
  • Bioinformatic analysis and luciferase reporter assays to confirm MTSS1 as a direct target of miR-411.

Main Results:

  • miR-411 expression was significantly upregulated in osteosarcoma patient serum and tissues compared to healthy controls.
  • Elevated miR-411 expression correlated with increased osteosarcoma cell proliferation and migration.
  • Metastasis suppressor protein 1 (MTSS1) was identified as a direct target of miR-411, with its expression inversely correlated with miR-411 levels in osteosarcoma tissues.

Conclusions:

  • miR-411 acts as an oncogene in osteosarcoma progression.
  • The oncogenic function of miR-411 is partly mediated by the inhibition of its direct target, MTSS1.
  • miR-411 represents a potential diagnostic biomarker and therapeutic target for osteosarcoma.