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Related Experiment Video

Updated: Feb 14, 2026

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

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Published on: June 13, 2020

Protein Turnover in Aging and Longevity.

Nathan Basisty¹, Jesse G Meyer¹, Birgit Schilling¹

¹The Buck Institute for Research on Aging, Novato, CA, USA.

|February 18, 2018

Summary

This summary is machine-generated.

Aging leads to a loss of proteostasis, impacting protein health. New proteomic methods now measure individual protein turnover in vivo, offering insights into aging and longevity.

Keywords:

aging mass spectrometry protein turnover proteostasis stable isotope labeling

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Area of Science:

Gerontology
Molecular Biology
Proteomics

Background:

Progressive loss of proteostasis is a key feature of aging.
This decline affects crucial cellular processes like autophagy and protein degradation.
Traditional methods assessed proteostasis through process changes or bulk proteome analysis.

Purpose of the Study:

To review the role of proteostasis in aging and longevity.
To focus on proteomic methods for measuring protein turnover in aging models.
To highlight insights gained from these proteomic studies.

Main Methods:

Utilizing stable-isotope labeling and mass spectrometry.
Employing specialized software for data analysis.
Measuring individual protein turnover directly in vivo.

Main Results:

Recent advances enable comprehensive measurement of individual protein turnover.
Proteomic methodologies have been applied to aging and longevity studies.
These methods provide detailed insights into age-related proteostasis decline.

Conclusions:

Proteostasis decline is central to aging.
Advanced proteomic techniques are crucial for understanding protein dynamics in aging.
In vivo protein turnover measurements offer novel perspectives on longevity research.