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Carboxymethyl konjac glucomannan - crosslinked chitosan sponges for wound dressing
Yu Xie1, Zeng-Xing Yi1, Jian-Xun Wang2
1College of Chemistry and Bio-Engineering, Yichun University, Yichun 336000, China; Joint Research Center of Qingdao Institute of Bioenergy and Bioprocess Technology of Chinese Academy of Sciences and Yichun University, Yichun 336000, China.
Abstract:
In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan. Then CMKGM and chitosan (CS) were crosslinked and freeze-dried to prepare CMKGM/CS composite sponges with different proportions. The structure and micromorphology of CMKGM/CS sponges were investigated by FTIR spectroscopy and SEM. The SEM results showed that the pore structure of the composite sponge gradually increased with the increase of CMKGM content. To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed. The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration. Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
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