Chronic exposure to inhaled, traffic-related nitrogen dioxide and a blunted cortisol response in adolescents

Chronic health effects of traffic-related air pollution, like nitrogen dioxide (NO₂), are well-documented. Animal models suggested that NO₂ exposures dysregulate cortisol function.

We evaluated the association between traffic-related NO₂ exposure and adolescent human cortisol concentrations, utilizing measures of the cortisol diurnal slope.

140 adolescents provided repeated salivary cortisol samples throughout one day. We built a land use regression model to estimate chronic NO₂ exposures based on home and school addresses. We then generated model-based estimates of the association between cortisol and NO₂ exposure one year prior to cortisol sampling, examining changes in cortisol diurnal slope. The final model was adjusted other criteria pollutants, measures of psychosocial stress, anthropometry, and other demographic and covariates.

We observed a decrease in diurnal slope in cortisol for adolescents exposed to the estimated 75th percentile of ambient NO₂ (high exposure) relative to those exposed at the 25th percentile (low exposure). For a highly exposed adolescent, the log cortisol was lower by 0.06 µg/dl at waking (95% CI: -0.15, 0.02), 0.07 µg/dl at 30 min post waking (95% CI: -0.15, 0.02), and higher by 0.05 µg/dl at bedtime (95% CI: 0.05, 0.15), compared to a low exposed adolescent. For an additional interquartile range of exposure, the model-based predicted diurnal slope significantly decreased by 0.12 (95% CI: -0.23, -0.01).

In adolescents, we found that increased, chronic exposure to NO₂ and the mixture of pollutants from traffic sources was associated with a flattened diurnal slope of cortisol, a marker of an abnormal cortisol response which we hypothesize may be a mechanism through which air pollution may affect respiratory function and asthma in adolescents.