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Updated: Feb 14, 2026

Nanopore DNA Sequencing for Metagenomic Soil Analysis
Published on: December 14, 2017
The soil microbiome-from metagenomics to metaphenomics
Janet K Jansson1, Kirsten S Hofmockel2
1Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA 99352, United States.
Abstract:
Soil microorganisms carry out important processes, including support of plant growth and cycling of carbon and other nutrients. However, the majority of soil microbes have not yet been isolated and their functions are largely unknown. Although metagenomic sequencing reveals microbial identities and functional gene information, it includes DNA from microbes with vastly varying physiological states. Therefore, metagenomics is only predictive of community functional potential. We posit that the next frontier lies in understanding the metaphenome, the product of the combined genetic potential of the microbiome and available resources. Here we describe examples of opportunities towards gaining understanding of the soil metaphenome.
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