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Atrial natriuretic factor in human plasma.

J Gutkowska, R Bonan, D Roy

    Biochemical and Biophysical Research Communications
    |August 29, 1986
    PubMed
    Summary
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    A new radioimmunoassay (RIA) accurately measures atrial natriuretic factor (ANF) in human plasma. This method revealed significantly higher ANF levels in coronary sinus blood compared to peripheral circulation.

    Area of Science:

    • Endocrinology
    • Cardiovascular Physiology
    • Analytical Chemistry

    Background:

    • Atrial natriuretic factor (ANF) plays a crucial role in cardiovascular homeostasis.
    • Accurate measurement of ANF in biological samples is essential for understanding its physiological functions.
    • Previous methods for ANF quantification may lack sensitivity or reproducibility.

    Purpose of the Study:

    • To develop and validate a sensitive and reproducible radioimmunoassay (RIA) for human plasma ANF.
    • To compare ANF concentrations in peripheral circulation versus coronary sinus blood.
    • To assess the correlation between a direct RIA and a prior extraction method.

    Main Methods:

    • Development of a radioimmunoassay (RIA) for immunoreactive ANF.
    • Plasma extraction using Sep-Pak cartridges with a specific buffer and acetonitrile.

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  • Assay sensitivity determination and assessment of inter- and intra-assay coefficients of variance.
  • Analysis of ANF levels in healthy subjects and coronary sinus blood.
  • High-performance liquid chromatography (HPLC) for elution profile analysis.

    • Main Results:

    • A sensitive RIA with a detection limit of 3.9 pg/ml was established.
    • Normal ANF levels in healthy subjects were 11.9 +/- 1.3 pg/ml.
    • Significantly higher ANF concentrations (6-37 times greater) were observed in coronary sinus blood.
    • Good correlation (r=0.91) was found between direct RIA and extraction method for ANF > 100 pg/ml.
    • HPLC revealed similar, complex elution profiles for peripheral and coronary sinus plasma extracts.

    Conclusions:

    • The developed RIA provides a reproducible and sensitive method for quantifying human plasma ANF.
    • Coronary sinus blood exhibits markedly elevated ANF levels, suggesting local cardiac production and release.
    • The direct RIA is reliable for higher ANF concentrations, while extraction is necessary for lower levels.