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Updated: Feb 14, 2026

Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(ε-caprolactone) Electrospun Yarns
Published on: April 10, 2015
Nanostructured electrospun nonwovens of poly(ε-caprolactone)/quaternized chitosan for potential biomedical
Danilo Martins Dos Santos1, Ilaiáli Souza Leite2, Andrea de Lacerda Bukzem1
1Sao Carlos Institute of Chemistry/University of Sao Paulo, Av. Trabalhador sao-carlense, 400-13566-590, Sao Carlos, São Paulo, Brazil.
Abstract:
Blend solutions of poly(ε-caprolactone) (PCL) and N-(2-hydroxy)-propyl-3-trimethylammonium chitosan chloride (QCh) were successfully electrospun. The weight ratio PCL/QCh ranged in the interval 95/5-70/30 while two QCh samples were used, namely QCh1 (DQ¯ = 47.3%; DPv¯ = 2218) and QCh2 (DQ¯ = 71.1%; DPv¯ = 1427). According to the characteristics of QCh derivative and to the QCh content on the resulting PCL/QCh nonwoven, the nanofibers displayed different average diameter (175 nm-415 nm), and the nonwovens exhibited variable porosity (57.0%-81.6%), swelling capacity (175%-425%) and water vapor transmission rate (1600 g m-2 24 h-2500 g m-2 24 h). The surface hydrophilicity of nonwovens increases with increasing QCh content, favoring fibroblast (HDFn) adhesion and spreading. Tensile tests revealed that the nonwovens present a good balance between elasticity and strength under both dry and hydrated state. Results indicate that the PCL/QCh electrospun nonwovens are new nanofibers-based biomaterials potentially useful as wound dressings.
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