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A multilayer coil in type-I counter-current chromatography.

Yi Yang1, Jiao Yang2, Chen Fang3

  • 1School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China; Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 5D18, Bethesda, MD 20892, USA; Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 10 Center Drive, Building 10, Room B2C315, Bethesda, MD 20892, USA.

Journal of Chromatography. A
|February 20, 2018
PubMed
Summary

A new multilayer coil model for type-I counter-current chromatography simplifies column manufacturing and broadens applications. This chromatography system shows high potential for various separation tasks.

Keywords:
DNP-amino acidsDipeptidesMultilayer coilType-I counter-current chromatography

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Area of Science:

  • Chromatography
  • Separation Science
  • Analytical Chemistry

Background:

  • Counter-current chromatography (CCC) is a liquid-liquid partition chromatography technique.
  • Traditional CCC column manufacturing can be complex and limit application scope.
  • Novel column designs are needed to enhance CCC performance and versatility.

Purpose of the Study:

  • To develop a novel model of type-I counter-current chromatography (CCC) using a multilayer coil.
  • To simplify the manufacturing process of CCC separation columns.
  • To expand the application range of type-I CCC.

Main Methods:

  • A multilayer coil was designed and employed as the separation column for type-I CCC.
  • Chromatographic performance was assessed by measuring stationary phase retention (Sf), theoretical plates (N), and peak resolution (Rs).
  • Experiments utilized dipeptides and DNP-amino acids with two solvent systems: 1-butanol-acetic acid-water (BAW) and hexane-ethyl acetate-methanol-0.1 M HCl (HEMW).

Main Results:

  • Optimal performance was achieved at a revolution speed of 200 rpm.
  • Lower mobile phase flow rates positively influenced stationary phase retention and peak resolution.
  • The developed multilayer coil CCC system demonstrated effective separation of test analytes.

Conclusions:

  • The novel multilayer coil CCC system offers a simplified manufacturing approach.
  • This new CCC model exhibits a broad application potential in separation science.
  • The system's performance is sensitive to operational parameters like revolution speed and flow rate.