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Increased proliferative activity in selenium-deficient mouse liver.

R Otter, M Goldberg, S Vogl

    Chemico-Biological Interactions
    |October 1, 1986
    PubMed
    Summary

    Selenium deficiency severely impacts mouse liver enzymes but enhances DNA replication during cell proliferation. This suggests an emergency response mechanism in selenium-depleted (Se-) livers when cell division is stimulated.

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    Area of Science:

    • Biochemistry
    • Nutritional Science
    • Cell Biology

    Background:

    • Selenium is an essential trace element crucial for various physiological processes.
    • Selenium deficiency can lead to significant alterations in enzyme activity and cellular function.
    • Understanding the role of selenium in liver cell proliferation is vital for nutritional and biomedical research.

    Purpose of the Study:

    • To investigate the effects of selenium deficiency on liver enzyme activities and DNA synthesis in mice.
    • To determine if selenium deficiency influences the rate of liver cell proliferation under stimulated conditions.

    Main Methods:

    • Male albino NMRI mice were divided into two groups: selenium-deficient (Se-) and selenium-supplemented (Se+).
    • Diets were maintained for at least 2 months, with Se- group receiving <10 ppb Se and Se+ group receiving 330 ppb Se.

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  • Liver enzyme activities, basal DNA synthesis, and DNA replication rates post-hepatopoietin or partial hepatectomy were measured.

    • Main Results:

    • Selenium-deficient mice exhibited multiple modulations in liver enzyme activities, confirming a severe Se- state.
    • No significant difference in basal DNA synthesis rates was observed between Se- and Se+ groups.
    • Induced liver cell proliferation (via hepatopoietin or partial hepatectomy) resulted in approximately a 3-fold increase in DNA replication rates in Se- mice compared to controls.

    Conclusions:

    • Selenium deficiency profoundly affects liver enzyme profiles.
    • While basal DNA synthesis is unaffected, selenium deficiency enhances liver cell proliferation rates during stimulated regeneration.
    • This enhanced proliferative activity in selenium-deficient mouse livers may represent an adaptive emergency response.