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High throughput imaging cytometer with acoustic focussing.

Robert Zmijan1, Umesh S Jonnalagadda1, Dario Carugo1

  • 1Engineering Sciences, Faculty of Engineering and the Environment, University of Southampton, Southampton, SO17 1BJ, UK.

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|February 20, 2018
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Summary

This study introduces an acoustic levitation imaging flow cytometer for high-throughput cell analysis. The novel system achieves high resolution imaging of thousands of cells per frame, advancing diagnostic capabilities.

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Area of Science:

  • Biomedical Engineering
  • Optical Physics
  • Cell Biology

Background:

  • Traditional flow cytometry systems are limited in throughput and imaging resolution.
  • Previous ultrasonic focusing techniques were primarily for 1D cytometry.

Purpose of the Study:

  • To develop a high-throughput imaging flow cytometer using acoustic levitation.
  • To integrate acoustic focusing with high-resolution imaging for enhanced cell analysis.

Main Methods:

  • Utilized acoustic levitation to form cells into a sheet structure for imaging.
  • Employed a galvo mirror for real-time image tracking of moving cells.
  • Integrated a high-resolution, low-noise CMOS camera for image acquisition.

Main Results:

  • Achieved high-resolution imaging of thousands of cells per frame.
  • Demonstrated a throughput of 208,000 beads per second at 80 frames per second.
  • Investigated factors influencing motion blur and throughput, showing potential for improvement with device length.

Conclusions:

  • Acoustic levitation enables a significant advancement in imaging flow cytometry throughput and capability.
  • The developed system offers new diagnostic possibilities beyond current technologies.
  • Further optimization can enhance throughput for cell-based analyses.