Related Experiment Video
Updated: Feb 14, 2026
Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening
Published on: February 17, 2018
[Micro-CT evaluating inhibitory effect of zoledronic acid on polyethylene particle-induced periprosthetic osteolysis
Lian-Guo Wu, Feng-Qing Wu, Hua Chen1
1Department of Orthopaedics, the Third Affiliated Hospital of Zhejiang University of Chinese Medical, Hangzhou 310005, Zhejiang, China; mdwu8535@126.com.
Objective:
To observe inhibitory effect of zoledronic acid on polyethylene particle-induced periprosthetic osteolysis model.
Methods:
Thirty male adult specific-pathogen-free SD rats (weighted 250 to 300 g) were randomly divided into sham group, model group, and zoledronic acid group, 10 in each group. Modeling were building by titanium screws and polyethylene particles implanted into right femur of rats, sham group and model group were performed hypodermic injection by 0.9% saline with 2 ml/kg, zoledronic acid with 0.1 ml/mg were injected into zoledronic acid group, once a week for 8 weeks. After 8 weeks, right femur specimens were drawn and used to san microstructure of femoral cancellous bone in rats model was examined by Micro-CT, and the images were treated with three-dimension reconstruction and analysis software BMD, BV/TV, Tb.N, Tb.Th, SMI, BS/BV, Tb.Sp and Tb.Pf and other parameters include.
Results:
According to Micro-CT three-dimensional imaging, BMD in model group was significantly decreased than sham group, bone microstructure damage was serious, bone trabecula changed thinning continuity; while bone microstructure was obviously improved compared with model group and zoledronic acid group. After analyzing Micro-CT parameters of femur microstructure, BMD in model group(0.081±0.020) was significantly decreased than control group(0.160±0.018) and zoledronic acid group(0.125±0.012); BV/TV in model group (10.563±1.070) was obviously lower than control group(27.935±1.834) and zoledronic acid group(14.559±1.258); Tb.N in model group (1.005±0.165) was lower than control group(2.058±0.108) and zoledronic acid group(1.515±0.126); while Tb.Th in model group (0.075±0.016) was decreased than control group(0.158±0.016) and zoledronic acid group(0.124±0.011). Meanwhile, SMI in model group(1.817±0.127) was significantly higher than control group(1.104±0.120) and zoledronic acid group(1.547±0.122); BS/BV in model group(35.784±1.650) was higher than control group(21.506±2.771) and zoledronic acid group(30.399±2.730); Tb.Sp in model group(0.735±0.107) was higher than control group(0.423±0.057) and zoledronic acid group(0.577±0.082), TB.Pf in model group(9.088±1.283) was higher than control group(2.447±0.703) and zoledronic acid group(5.862±1.042).
Conclusions:
Zoledronic acid could change bone microstructure of rats to inhibit polyethylene particle-induced bone solution, which provides a scientific basis for prevent bone solution by zoledronic acid as a therapeutic intervention.
Insights
Zoledronic acid effectively inhibits polyethylene particle-induced osteolysis in a rat model. This study demonstrates its potential to prevent bone loss and improve bone microstructure, offering a therapeutic intervention for periprosthetic bone solution.
Area of Science:
- Orthopedics and Biomaterials Science
- Pharmacology and Toxicology
- Skeletal Biology and Disease
Background:
- Periprosthetic osteolysis, often induced by polyethylene particles, is a significant complication of joint replacement surgery.
- Understanding the mechanisms of osteolysis is crucial for developing effective preventative and therapeutic strategies.
Purpose of the Study:
- To investigate the inhibitory effects of zoledronic acid on polyethylene particle-induced periprosthetic osteolysis.
- To evaluate the impact of zoledronic acid on bone microstructure in a rat model.
Main Methods:
- A rat model of periprosthetic osteolysis was established using polyethylene particles and titanium screws in the femur.
- Rats were divided into sham, model, and zoledronic acid treatment groups.
- Micro-computed tomography (Micro-CT) was used to analyze bone microstructure and parameters like bone mineral density (BMD) and bone volume/total volume (BV/TV).
Main Results:
- Polyethylene particle implantation led to significantly decreased BMD, reduced bone volume, and deteriorated bone microstructure in the model group compared to the sham group.
- Zoledronic acid treatment notably improved bone microstructure, increased BMD, and favorably altered bone parameters (BV/TV, Tb.N, Tb.Th) compared to the model group.
- Significant differences were observed in bone structure indices including SMI, BS/BV, Tb.Sp, and Tb.Pf between the groups, with zoledronic acid showing a protective effect.
Conclusions:
- Zoledronic acid demonstrates a significant inhibitory effect on polyethylene particle-induced osteolysis.
- The drug improves bone microstructure and offers a potential therapeutic intervention for preventing bone loss associated with periprosthetic osteolysis.
Related Concept Videos
03:37Subatomic Particles
02:10The Nucleosome Core Particle
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
01:13Induced-fit Model
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
01:29Self-Evaluation Maintenance Model
01:29Excitatory and Inhibitory Effects of Neurotransmitters
02:33Lewis Acids and Bases
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...