Related Experiment Videos
The inhibitory effect of granulocyte factor in mixed lymphocyte reaction
Abstract:
The polymorphonuclear leukocytes (PMNLs) depress mixed leukocyte reaction (MLR) in vitro. The active factor involved in inhibition was found to be localized in the specific granules of PMNL. The adherent granulocytes secreted granulocyte factor (GF) which also diminished MLR. The functional activity of GF is reflected by inhibition of responding cell proliferation exclusively during induction of MLR; moreover, GF depresses generation of the specific suppressor cells associated with MLR. The effect of GF is modified using specific antisera.
Insights
Polymorphonuclear leukocytes (PMNLs) inhibit mixed leukocyte reactions (MLR) via a factor in their granules. This granulocyte factor (GF) suppresses responding cell proliferation and suppressor cell generation during MLR.
Area of Science:
- Immunology
- Cell Biology
Background:
- Polymorphonuclear leukocytes (PMNLs) play a role in immune responses.
- The mixed leukocyte reaction (MLR) is a key model for studying T-cell activation and allo-recognition.
Purpose of the Study:
- To identify the inhibitory factor from PMNLs that affects MLR.
- To characterize the mechanism of action of this factor on MLR.
Main Methods:
- In vitro mixed leukocyte reaction assays.
- Isolation and characterization of factors from PMNL specific granules.
- Assessment of cell proliferation and suppressor cell generation.
Main Results:
- PMNLs were found to inhibit MLR in vitro.
- An active inhibitory factor, granulocyte factor (GF), was localized to PMNL specific granules.
- GF inhibited responding cell proliferation specifically during MLR induction.
- GF also suppressed the generation of MLR-associated suppressor cells.
- The activity of GF was modulated by specific antisera.
Conclusions:
- Granulocyte factor (GF) secreted by PMNLs is responsible for the inhibition of MLR.
- GF acts by suppressing T-cell proliferation and suppressor cell development during the initial phase of MLR.