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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Potato Virus X-Based microRNA Silencing VbMS In Potato.
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Sphingobacterium solani sp. nov., isolated from potato stems.

Xinbin Niu1, Weiguo Cui2, Man Cui2

  • 1Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, PR China.

International Journal of Systematic and Evolutionary Microbiology
|February 21, 2018
PubMed
Summary

A novel bacterium, Sphingobacterium solani sp. nov., was isolated from potato stems. This discovery expands the known diversity within the Sphingobacterium genus, offering new insights into plant-associated microbial communities.

Keywords:
Sphingobacteriumnew speciesphylogenetic analysis

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Area of Science:

  • Microbiology
  • Bacteriology
  • Plant Pathology

Background:

  • Plant-associated bacteria play crucial roles in soil health and plant physiology.
  • The genus Sphingobacterium comprises Gram-negative bacteria with diverse ecological niches.
  • Understanding novel bacterial species aids in comprehending microbial ecosystems and their interactions.

Purpose of the Study:

  • To isolate and characterize a novel bacterial strain from potato stems.
  • To determine the phylogenetic and genotypic relationship of the isolate to known species.
  • To formally propose a new species within the genus Sphingobacterium.

Main Methods:

  • Isolation and cultivation of bacteria from potato stems.
  • Gram staining, motility, and spore formation tests.
  • Physiological characterization (temperature, pH, NaCl tolerance).
  • Phylogenetic analysis using 16S rRNA gene sequences.
  • Analysis of cellular fatty acids and polar lipids.
  • Determination of DNA G+C content and in silico DNA-DNA reassociation values.

Main Results:

  • A novel Gram-negative bacterium, strain MLS-26-JM13-11T, was isolated.
  • The strain exhibited optimal growth at 30°C, pH 7.2, and 1.0% NaCl.
  • Phylogenetic analysis showed highest similarity to Sphingobacterium bambusae and Sphingobacterium griseoflavum.
  • Genomic analyses (ANI and in silico DDH) confirmed its status as a distinct species.
  • Major fatty acids included iso-C15:0 and summed feature 3.

Conclusions:

  • Strain MLS-26-JM13-11T represents a novel species of the genus Sphingobacterium.
  • The proposed name for this new species is Sphingobacterium solani sp. nov.
  • This finding contributes to the taxonomic diversity of plant-associated bacteria.