Vibrio fujianensis sp. nov., isolated from aquaculture water

A Gram-stain-negative, facultatively anaerobic strain, designated FJ201301^T, was isolated from aquaculture water collected from Fujian province, China. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain FJ201301^T belonged to the genus Vibrio, formed a distinct cluster with Vibriocincinnatiensis ATCC 35912^T and shared the highest similarity with Vibriosalilacus CGMCC 1.12427^T. A 15 bp insertion found in the 16S rRNA gene was a significant marker that distinguished strain FJ201301^T from several phylogenetic neighbours (e.g. V. cincinnatiensis). Multilocus sequence analysis of eight genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA and topA; concatenated 4135 bp sequence) showed that, forming a long and independent phylogenetic branch, strain FJ201301^T clustered with V. cincinnatiensis ATCC 35912^T, Vibrioinjenensis KCTC 32233^T and Vibriometschnikovii CIP 69.14^T clearly separated from V. salilacus CGMCC 1.12427^T. Furthermore, the highest in silico DNA-DNA hybridization and average nucleotide identity values between strain FJ201301^T and the closest related species were 26.3 and 83.1 % with V. cincinnatiensis ATCC 35912^T, less than the proposed cutoff levels for species delineation, i.e. 70 and 95 %, respectively. Biochemical, sequence and genomic analysis suggested the designation of strain FJ201301^T representing a novel species of the genus Vibrio, for which the name Vibrio fujianensis sp. nov. is proposed. The type strain is FJ201301^T (=DSM 104687^T=CGMCC 1.16099^T).