Paenibacillus mobilis sp. nov., a Gram-stain-negative bacterium isolated from soil

A novel Gram-stain-negative bacterium, designated strain S8^T, was isolated from a soil sample obtained in Gyeonggi Province, Republic of Korea. Cells of strain S8^T were endospore-forming, motile by means of peritrichous flagella, and rod-shaped. S8^T colonies were round, convex, wavy and white. Strain S8^T grew optimally at 37 °C, pH 6-8, and up to 2.0 % (w/v) NaCl. Based on 16S rRNA gene sequence similarity, strain S8^T was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84^T and Paenibacillus physcomitrellae XB^T (98.8 and 97.1 % sequence similarity). The DNA G+C content of the novel strain was 53.1±0.3 mol%. Strain S8^T contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids. The major fatty acid was anteiso-branched C15 : 0. The quinone was menaquinone MK-7. The peptidoglycan of strain S8^T contained meso-diaminopimelic acid. The DNA-DNA hybridization values of strain S8^T with P. yonginensis KCTC 33428^T and P. physcomitrellae DSM 29851^T were 44 % and 32 %, respectively. Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8^T (=KCTC 33848^T=JCM 31672^T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp. nov. is proposed.