Phyllobacterium salinisoli sp. nov., isolated from a Lotus lancerottensis root nodule in saline soil from Lanzarote

A Gram-negative rod, designated strain LLAN61^T, was isolated from a root nodule of Lotus lancerottensis growing in a saline soil sample from Lanzarote (Canary Islands). The strain grew optimally at 0.5 % (w/v) NaCl and tolerated up to 3.5 %. The 16S rRNA gene sequence analysis showed that strain LLAN61^T belonged to genus Phyllobacterium and that Phyllobacteriumleguminum ORS 1419^T and Phyllobacteriummyrsinacearum IAM 13584^T are the closest related species with 97.93 and 97.86% similarity values, respectively. In the atpD phylogeny, P. leguminum ORS 1419^T and P. myrsinacearum ATCC 43591^T, sharing similarities of 87.6 and 85.8% respectively, were also the closest species to strain LLAN61^T. DNA-DNA hybridization showed an average value of 21 % between strain LLAN61^T and P. leguminum LMG 22833^T, and 6 % with P. myrsinacearum ATCC 43590^T. The predominant fatty acids were C19 : 0 cyclo ω8c and C18 : 1ω6c/C18 : 1ω7c (summed feature 8). The DNA G+C content was 58.0 mol%. Strain LLAN61^T differed from its closest relatives in some culture conditions and in assimilation of several carbon sources. Based upon the results of phylogeny, DNA-DNA hybridization, phenotypic tests and fatty acid analysis, this strain should be classified as a novel species of Phyllobacterium for which the name Phyllobacterium salinisoli sp. nov. is proposed (type strain LLAN61^T=LMG 30173^T = CECT 9417^T).