Paenibacillus translucens sp. nov., isolated from tidal flat sediment

A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11^T, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea. Strain CJ11^T grew optimally on R2A at 30 °C and pH 7.0. Sequencing results of the 16S rRNA gene revealed that strain CJ11^T possesses two copies of the 16S rRNA gene varying at five nucleotide positions. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11^T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691^T (99.36-99.15 % similarity). DNA-DNA relatedness levels of strain CJ11^T was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691^T. The G+C content of the genomic DNA was 51.0 mol%. Strain CJ11^Tcontained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major isoprenoid quinone was menaquinone-7. The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids. On the basis of the polyphasic taxonomic study, strain CJ11^T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp. nov. is proposed. The type strain is CJ11^T (=KACC 19304^T=JCM 32080^T).