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Updated: Feb 14, 2026

Oligopeptide Competition Assay for Phosphorylation Site Determination
Published on: May 18, 2017
Does the Abiotic Formation of Oligopeptides on TiO₂ Nanoparticles Require Special Catalytic Sites? Apparently Not
Marco Fabbiani1, Marco Pazzi2, Marco Vincenti2
1Department of Science and High Technology and INSTM, University of Insubria, via Valleggio 9, 22100 Como, Italy.
Abstract:
The oligomerization of non-activated amino acids catalyzed by nanostrucrured mineral oxide surfaces holds promises as a sustainable route for the industrial production of polypeptides. To analyze the influence of the surface type on the catalytic process, we performed, via a mild Chemical Vapor Deposition approach, the oligomerization of Glycine on two samples of TiO2 nanoparticles characterized by different relative amounts of defective surface terminations. Based on infrared spectroscopy and mass spectrometry data, we show herein that the formation of peptide bonds on titania nanoparticles does not require highly energetic surface terminations, but can occur also on the most abundant and thermodynamically most stable {101} facets of nanosized anatase.
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