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Published on: January 30, 2020
First report of a cross-kingdom pathogenic bacterium, Achromobacter xylosoxidans isolated from stipe-rot Coprinus
Luona Ye1, Mengpei Guo1, Pengfei Ren2
1Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan, 430070, China.
Abstract:
Coprinus comatus is an edible mushroom widely cultivated in China as a delicious food. Various diseases have occurred on C. comatus with the cultivated area increasing. In this study, the pathogenic bacterium JTG-B1, identified as Achromobacter xylosoxidans by 16S rDNA and nrdA gene sequencing, was isolated from edible mushroom Coprinus comatus with serious rot disease on its stipe. A. xylosoxidans has been confirmed as an important opportunistic human pathogenic bacterium and has been isolated from respiratory samples from cystic fibrosis. It is widely distributed in the environment. Here, we first report that fungi can also serve as a host for A. xylosoxidans. We confirmed that it can cross-kingdom infect between animals (mice) and fungi (C. comatus). The results of pathogenicity tests, physiological, biochemical and genotyping analysis of A. xylosoxidans from different hosts suggested that different strain of A. xylosoxidans may have pathogenicity differentiation. A. xylosoxidans not only is pathogenic to C. comatus but also may threaten human health.
Insights
A pathogenic bacterium, Achromobacter xylosoxidans, was found to infect the edible mushroom Coprinus comatus. This bacterium can also infect animals, posing a potential threat to both fungi and human health.
Area of Science:
- Mycology
- Bacteriology
- Pathogen Research
Background:
- Coprinus comatus (edible mushroom) cultivation is increasing in China.
- Diseases affecting C. comatus are becoming more prevalent.
- Achromobacter xylosoxidans is a known opportunistic human pathogen.
Purpose of the Study:
- To identify the pathogen causing rot disease in Coprinus comatus.
- To investigate the host range and pathogenicity of the isolated bacterium.
- To explore potential cross-kingdom infection between fungi and animals.
Main Methods:
- Isolation and identification of pathogenic bacterium JTG-B1 using 16S rDNA and nrdA gene sequencing.
- Pathogenicity tests on Coprinus comatus and mice.
- Physiological, biochemical, and genotyping analysis of A. xylosoxidans strains.
Main Results:
- The isolated bacterium was identified as Achromobacter xylosoxidans.
- A. xylosoxidans was confirmed as pathogenic to C. comatus, causing stipe rot.
- Cross-kingdom infection was demonstrated between mice and C. comatus.
- Pathogenicity differentiation was observed among different strains of A. xylosoxidans.
Conclusions:
- Fungi, specifically Coprinus comatus, can serve as a host for Achromobacter xylosoxidans.
- A. xylosoxidans poses a dual threat, affecting edible mushrooms and potentially human health.
- Further research is needed to understand the pathogenicity differentiation of A. xylosoxidans strains.
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