Bioaccumulation and effects of titanium dioxide nanoparticles and bulk in the clam Ruditapes philippinarum

Biochemical and cellular responses to low concentrations of TiO₂ nanoparticles (nTiO_2, 1 and 10 μg/L) and bulk (bTiO₂, 10 μg/L) were evaluated in gills, digestive gland and haemolymph of the clam Ruditapes philippinarum after1, 3 and 7 days' exposure. At 7 days, titanium content was determined in gills and digestive gland. nTiO₂ significantly increased antioxidant enzyme activities in both tissues, and lipid peroxidation in digestive gland at 10 μg/L only, and affected glutathione S-transferase activity. Slighter variations were observed in bTiO₂-treated clams. A significant Ti bioaccumulation was detected in both gills and digestive gland of 10 μg nTiO₂/L-exposed clams. In haemolymph, nTiO₂ affected total haemocyte count, haemocyte proliferation, haemocyte diameter and volume, and induced DNA damage. Overall, this study demonstrated that TiO₂ alters most of the biomarkers measured in clams, although responses were differently modulated depending on tissues and exposure conditions, and indicated that nTiO₂ can be accumulated by bivalves, suggesting a potential risk for filter-feeding animals.