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Updated: Feb 14, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
A dual-mode mobile phone microscope using the onboard camera flash and ambient light
A Orth1, E R Wilson2, J G Thompson3
1ARC Centre of Excellence for Nanoscale BioPhotonics, School of Science, RMIT University, Melbourne, Australia. antony.orth@rmit.edu.au.
Abstract:
Mobile phone microscopes are a natural platform for point-of-care imaging, but current solutions require an externally powered illumination source, thereby adding bulk and cost. We present a mobile phone microscope that uses the internal flash or sunlight as the illumination source, thereby reducing complexity whilst maintaining functionality and performance. The microscope is capable of both brightfield and darkfield imaging modes, enabling microscopic visualisation of samples ranging from plant to mammalian cells. We describe the microscope design principles, assembly process, and demonstrate its imaging capabilities through the visualisation of unlabelled cell nuclei to observing the motility of cattle sperm and zooplankton.
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