Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Base-Promoted α-Halogenation of Aldehydes and Ketones00:51

Base-Promoted α-Halogenation of Aldehydes and Ketones

4.2K
α-Halogenation of aldehydes and ketones is a reaction involving the substitution of α hydrogens with halogens in the presence of a base.  The reaction begins with the abstraction of  α hydrogen by the base to produce a nucleophilic enolate ion. This intermediate undergoes a subsequent nucleophilic substitution with the halogen to produce a monohalogenated carbonyl compound. If the starting substrate has more than one α hydrogen, it is difficult to stop the reaction...
4.2K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

19.0K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
19.0K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

4.0K
4.0K
The Extracellular Matrix01:42

The Extracellular Matrix

89.5K
Overview
89.5K
The Extracellular Matrix01:29

The Extracellular Matrix

12.5K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
12.5K
Dimensional Analysis03:40

Dimensional Analysis

65.3K
Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units.
Conversion Factors and Dimensional Analysis
The unit...
65.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is traditional Chinese medicine service accessibility associated with health inequality? Evidence from the distribution of health capital among rural residents in China.

International journal for equity in health·2026
Same author

The Target ALS Global Natural History Study: Cross-platform proteomics to accelerate biofluid biomarker and drug target discovery in amyotrophic lateral sclerosis.

medRxiv : the preprint server for health sciences·2026
Same author

Capturing Elusive Volatile Organics in Atmospheric Particles via Dual-Mode Solid-Phase Microextraction Coupled with Gas Chromatography-Orbitrap Mass Spectrometry.

Analytical chemistry·2026
Same author

Postmortem Evidence of Lymphatic Vessels Located at the Boundary of Central and Peripheral Nervous Systems in the Cervical Spine.

Cellular and molecular neurobiology·2026
Same author

AI-powered and manual assessment of tumor-infiltrating lymphocytes in early HER2-positive breast cancer in NSABP B-41.

NPJ breast cancer·2026
Same author

Cysteine-rich secreted protein OsCRRSP1 enhances rice defense through dual inhibition of pathogen and host catalases.

Plant communications·2026

Related Experiment Video

Updated: Feb 14, 2026

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
08:55

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Published on: July 6, 2022

2.5K

Three-Dimensional-Bioprinted Dopamine-Based Matrix for Promoting Neural Regeneration.

Xuan Zhou, Haitao Cui, Margaret Nowicki

  • 1Department of Mechanical Engineering , University of Maryland , Collage Park , Maryland 20742 , United States.

ACS Applied Materials & Interfaces
|February 21, 2018
PubMed
Summary

This study developed 3D-printed Gelatin methacrylate-dopamine scaffolds to enhance neural stem cell differentiation for nerve regeneration. The novel scaffolds promote neural network formation and gene expression, offering promising therapeutic potential.

Keywords:
3D bioprintingdopaminegelatin methacrylateneural stem cellsneural tissue engineering

More Related Videos

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy
05:20

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Published on: June 29, 2021

3.5K
Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter
08:40

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Published on: May 16, 2019

10.3K

Related Experiment Videos

Last Updated: Feb 14, 2026

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
08:55

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Published on: July 6, 2022

2.5K
Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy
05:20

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Published on: June 29, 2021

3.5K
Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter
08:40

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Published on: May 16, 2019

10.3K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Neuroscience

Background:

  • Central nerve repair faces challenges due to limited regeneration and fibrosis.
  • Current clinical solutions for nerve repair are inadequate.
  • 3D bioprinting offers a promising approach for creating artificial nerve scaffolds.

Purpose of the Study:

  • To fabricate 3D-bioprinted Gelatin methacrylate-dopamine (GelMA-DA) neural scaffolds.
  • To investigate the efficacy of GelMA-DA scaffolds in promoting neural stem cell (NSC) differentiation.
  • To evaluate the potential of these scaffolds for nerve regeneration.

Main Methods:

  • Custom stereolithography-based 3D bioprinting of GelMA-DA scaffolds.
  • Functionalization of GelMA with dopamine (DA) to create a printable ink.
  • Culturing neural stem cells (NSCs) on the 3D scaffolds.
  • Confocal microscopy and gene expression analysis (TUJ1, MAP2) to assess neural differentiation.

Main Results:

  • GelMA-DA scaffolds exhibited a porous, interconnected 3D structure supporting NSC growth.
  • Distinct neural networks formed on the GelMA-DA scaffolds.
  • Significant enhancement in neuron gene expression (TUJ1 and MAP2) was observed.

Conclusions:

  • 3D-printed GelMA-DA scaffolds effectively promote neural differentiation.
  • These customizable scaffolds provide a favorable microenvironment for neural stem cell development.
  • GelMA-DA scaffolds show significant promise for advancing future nerve repair and regeneration strategies.