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Related Experiment Videos

High-sensitivity radioimmunoassay screening method for fentanyl.

W E Woods, H H Tai, C Tai

    American Journal of Veterinary Research
    |October 1, 1986
    PubMed
    Summary

    A new radioiodinated fentanyl analog significantly enhances radioimmunoassay sensitivity for detecting fentanyl in horse urine. This highly sensitive assay allows for economical, large-scale screening of equine samples post-race.

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    Annals of the Royal College of Surgeons of England·2017

    Area of Science:

    • Analytical Chemistry
    • Pharmacology
    • Equine Sports Medicine

    Background:

    • Fentanyl is a potent synthetic opioid used in veterinary medicine.
    • Accurate and sensitive detection of fentanyl in equine samples is crucial for anti-doping efforts in horse racing.
    • Existing radioimmunoassays for fentanyl may lack the sensitivity required for detecting low-dose or early administration.

    Purpose of the Study:

    • To synthesize a radioiodinated analog of fentanyl.
    • To develop a highly sensitive radioimmunoassay for fentanyl detection in equine urine.
    • To evaluate the assay's performance for detecting fentanyl and related compounds in post-race horse urine samples.

    Main Methods:

    • Synthesis of a radioiodinated fentanyl analog.
    • Modification of a commercially available radioimmunoassay for fentanyl.

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  • Assay validation using equine urine samples spiked with fentanyl.
  • Detection limits and sensitivity assessment.

    • Main Results:

    • The modified radioimmunoassay demonstrated at least 100-fold greater sensitivity compared to the original assay.
    • Fentanyl concentrations as low as 1 pg/ml were detectable in equine urine.
    • Sub-pharmacological doses of fentanyl were detectable for over 96 hours.
    • The assay could detect sufentanil and its metabolites, albeit at lower efficiency.

    Conclusions:

    • A highly sensitive radioiodinated fentanyl analog enables a significantly improved radioimmunoassay for fentanyl detection.
    • This enhanced assay is suitable for economical, large-scale screening of equine urine samples, aiding in the detection of fentanyl misuse in horse racing.
    • The assay's sensitivity allows for the detection of fentanyl at doses and durations previously undetectable.