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Sensitive analytical method to quantify clindamycin in plasma and microdialysate samples: Application in a
N G Girondi1, F Barreto2, M C Pigatto1
1Pharmacokinetics Modeling Laboratory, PK/PD, Pharmaceutical Sciences Post-Graduation Program, Pharmacy College, Federal University of Rio Grande do Sul, Brazil.
Journal of Pharmaceutical and Biomedical Analysis
|February 21, 2018
Summary
This study developed a validated method to measure clindamycin in plasma and subcutaneous tissue, crucial for effective antibiotic prophylaxis against surgical site infections.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Infectious Diseases
Background:
- Clindamycin is essential for preventing surgical site infections.
- Therapeutic clindamycin concentrations in subcutaneous tissue are vital for prophylaxis efficacy.
- Monitoring drug levels at the incision site is necessary for optimal antibiotic use.
Purpose of the Study:
- To develop and validate a high-performance liquid chromatography-mass spectrometry method for quantifying clindamycin.
- To measure clindamycin concentrations in both plasma and subcutaneous microdialysate.
- To assess clindamycin penetration into subcutaneous tissue for improved antibiotic prophylaxis.
Main Methods:
- High-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).
- Reverse-phase C18 column chromatography with a gradient elution.
- Analysis of plasma and subcutaneous microdialysate samples in Wistar rats.
Main Results:
- The validated HPLC-MS method demonstrated selectivity, precision, and accuracy.
- Linearity was established for clindamycin detection in plasma (0.5-100 μg/mL) and microdialysate (25-1000 ng/mL).
- The method was successfully applied to a pharmacokinetic study of clindamycin.
Conclusions:
- A robust analytical method for determining clindamycin in plasma and subcutaneous tissue was established.
- This method enables the investigation of drug penetration at the surgical site.
- Findings support optimizing clindamycin dosing for effective surgical site infection prophylaxis.
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