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Species C Rotaviruses in Children with Diarrhea in India, 2010-2013: A Potentially Neglected Cause of Acute

Sudipta Bhat1, Jobin Jose Kattoor2, Yashpal Singh Malik3

  • 1Division of Biological Standardization, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India. sudiptabhat1991@gmail.com.

Pathogens (Basel, Switzerland)
|February 22, 2018
PubMed

Insights

Rotavirus C (RVC) is a significant cause of gastroenteritis in India. This study detected RVC in 31.93% of diarrheic children in Haldwani, suggesting potential zoonotic transmission and the need for integrated surveillance.

Area of Science:

  • Virology
  • Public Health
  • Epidemiology

Background:

  • Rotavirus C (RVC) is an emerging enteric pathogen causing severe gastroenteritis globally.
  • Limited surveillance data exists for RVC in India, with prevalence largely unknown outside outbreaks.
  • RVC belongs to the Reoviridae family, with diverse species affecting various mammals and birds.

Purpose of the Study:

  • To detect the prevalence of RVC in diarrheic children in Haldwani, Uttarakhand, India.
  • To perform phylogenetic analysis of identified RVC strains.
  • To investigate potential zoonotic transmission of RVC.

Main Methods:

  • Screening of 119 stool samples from diarrheic children using RVC VP6 gene-specific RT-PCR.
  • Phylogenetic analysis of the VP6 and NSP4 genes from a human RVC isolate (HuRVC/H28/2013/India).
  • Comparison of genetic sequences with known human and animal RVC strains.

Main Results:

  • 38 out of 119 samples (31.93%) tested positive for RVC, a higher incidence than previously reported in India.
  • Phylogenetic analysis revealed the Indian isolate belongs to genotypes I2, P2, and E2.
  • The VP6 gene showed high similarity to a human-like porcine RVC from India, suggesting zoonotic transmission.

Conclusions:

  • RVC is prevalent in diarrheic children in Haldwani, India.
  • Evidence suggests potential zoonotic transmission of RVC between animals and humans in India.
  • Integrated human and animal RVC surveillance under One Health platforms is crucial for understanding epidemiology and implementing control strategies.

Keywords:
IndiaNSP4 genesVP4VP6acute gastroenteritisphylogenetic analysisrotavirus Csequence analysis

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