Related Experiment Video

Updated: Feb 14, 2026

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
11:24

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Published on: March 25, 2015

10.5K

Zinc finger protein 598 inhibits cell survival by promoting UV-induced apoptosis

Qiaohong Yang1, Romi Gupta1

  • 1Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA.

Oncotarget
|February 22, 2018
PubMed

Insights

Zinc finger protein 598 (ZNF598) protects cancer cells from UV-induced apoptosis by upregulating apoptotic gene expression. Loss of ZNF598 confers resistance to UV-induced apoptosis, highlighting its role in cancer cell survival.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cellular Stress Response

Background:

  • Ultraviolet (UV) radiation is a significant inducer of DNA damage and apoptosis.
  • Cancer cells frequently develop mechanisms to evade apoptosis, contributing to tumor progression and treatment resistance.

Purpose of the Study:

  • To identify novel factors that confer resistance to UV-induced apoptosis in cancer cells.
  • To elucidate the role of Zinc finger protein 598 (ZNF598) in regulating UV-induced apoptosis.

Main Methods:

  • Genome-wide short-hairpin RNA (shRNA) screening to identify key regulators of UV-induced apoptosis.
  • Analysis of ZNF598 and ELK1 expression and phosphorylation levels following UV irradiation.
  • Assessment of apoptosis using annexin V staining and apoptotic gene expression analysis.

Main Results:

  • UV irradiation transcriptionally upregulates ZNF598 expression in cancer cells.
  • ZNF598 knockdown inhibits UV-induced apoptosis, while its ectopic expression promotes it.
  • UV-induced ZNF598 upregulation is dependent on ELK1 activation, and ELK1 knockdown also confers resistance to UV-induced apoptosis.

Conclusions:

  • ZNF598 is a critical mediator of UV-induced apoptosis, acting downstream of ELK1.
  • ZNF598 plays a protective role in cancer cells against UV-induced cell death.
  • Targeting ZNF598 may represent a therapeutic strategy to enhance cancer cell sensitivity to UV-based treatments.
Keywords:
UV-inducedapoptosisphenocopiesupregulationzinc finger protein

More Related Videos

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis12:55

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Published on: February 16, 2015

18.7K
Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis04:37

Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

Published on: February 24, 2011

12.5K

Related Concept Videos

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
19.0K
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
15.3K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.3K
Survival Curves01:18

Survival Curves

Survival curves are graphical representations that depict the survival experience of a population over time, offering an intuitive way to track the proportion of individuals who remain event-free at each time point. These curves are widely used in fields such as medicine, public health, and reliability engineering to visualize and compare survival probabilities across different groups or conditions.
The Kaplan-Meier estimator is the most common method for constructing survival curves. This...
745
Survival Tree01:19

Survival Tree

Survival trees are a non-parametric method used in survival analysis to model the relationship between a set of covariates and the time until an event of interest occurs, often referred to as the "time-to-event" or "survival time." This method is particularly useful when dealing with censored data, where the event has not occurred for some individuals by the end of the study period, or when the exact time of the event is unknown.
 Building a Survival Tree
Constructing a...
437
Muscles of the Forearm that Move the Hand and Fingers01:16

Muscles of the Forearm that Move the Hand and Fingers

The muscles of the forearm that move the wrist, hand, and digits are numerous and diverse. They can be classified into two groups based on their location and function — the anterior and posterior compartment muscles.
Anterior Compartment
The anterior compartment muscles originate from the humerus. They primarily function as flexors and are also known as flexor muscles. They typically insert on the carpals, metacarpals, and phalanges. The superficial layer includes the flexor carpi...
2.7K