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Updated: Feb 14, 2026

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
Use of Viable Cryopreserved Placental Membrane as an Adjunct to Facial Keloid Resection
Rishi J Gupta1, Stephen T Connelly1, Rebeka G Silva1
1Department of Oral Maxillofacial Surgery, San Francisco Veterans Affairs Medical Center, San Francisco, Calif.; Department of Oral Maxillofacial Surgery, University of California, San Francisco Medical Center, San Francisco, Calif.; and Department of Medical Affairs, Osiris Therapeutics, Inc., Columbia, Md.
Abstract:
Keloids are the physical manifestation of an exaggerated inflammatory response resulting in excess collagen deposition. The resulting fibroproliferative mass can be distressing for patients due to appearance, pruritus, and/or pain. Despite extensive research into the pathophysiology of keloid formation and the development of numerous treatments, keloids remain a challenge to treat. Even when the initial treatment is successful, a risk of recurrence remains. Basic science research into viable cryopreserved placental membranes and viable cryopreserved umbilical tissue has demonstrated their anti-inflammatory and anti-fibrotic effects, which may decrease keloid recurrence after excision. In this article, we present the first-reported case of viable cryopreserved placental membrane, with living mesenchymal stem cells, to treat a painful preauricular keloid in conjunction with surgical resection.
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