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The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats

Caipan Gong1, Li Li1, Chunmei Qin2

  • 1Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.

Journal of Diabetes Research
|February 22, 2018
PubMed
Abstract

Insights

Diabetic nephropathy with vascular calcification involves changes in smooth muscle and bone cell markers. The Notch1-RBP-Jk/Msx2 pathway activation appears to drive this vascular calcification progression.

Area of Science:

  • Nephrology
  • Cardiovascular Biology
  • Molecular Biology

Background:

  • Diabetic nephropathy (DN) is associated with vascular calcification.
  • Vascular calcification in DN involves alterations in vascular smooth muscle cell (VSMC) and osteogenic markers.
  • The role of the Notch1-RBP-Jk/Msx2 pathway in DN-related vascular calcification requires further investigation.

Purpose of the Study:

  • To investigate changes in VSMC and osteogenic markers in a rat model of DN with vascular calcification.
  • To explore the involvement of the Notch1-RBP-Jk/Msx2 signaling pathway in this condition.

Main Methods:

  • A rat model of diabetic nephropathy with vascular calcification was established using streptozotocin, vitamin D3, and nicotine.
  • Aortic tissue was analyzed for biochemical and histological changes.
  • Quantitative real-time polymerase chain reaction and immunohistochemical analysis were used to assess marker expression.

Main Results:

  • Model rats showed elevated serum calcium and phosphorus levels.
  • Aortic intima-media displayed mineral deposits, reduced VSMC markers, and increased osteogenic markers.
  • Expression of Notch1, RBP-Jk, Msx2, Jagged1, and N1-ICD significantly increased in model rats compared to controls.

Conclusions:

  • The Notch1-RBP-Jk/Msx2 signaling pathway is implicated in the development and progression of vascular calcification in diabetic nephropathy.
  • These findings highlight a potential therapeutic target for managing vascular complications in DN.

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