Related Experiment Video
Updated: Feb 14, 2026

Murine Dermal Fibroblast Isolation by FACS
Published on: January 7, 2016
Direct electrospinning of poly(vinyl butyral) onto human dermal fibroblasts using a portable device
Chih-Yao Chui1, Pierre-Alexis Mouthuy2, Hua Ye3
1Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Old Road Campus, Oxford, OX3 7DQ, UK.
Objective:
To demonstrate that uniform poly(vinyl butyral) (PVB) fibres can be safely electrospun onto a monolayer of human dermal fibroblasts using a portable device.
Results:
PVB in solvent mixtures containing various amounts of ethanol and water was electrospun. Six percent (weight-to-volume ratio) PVB in a 9:1 ethanol:water ratio was the solution with the highest content in water that could be electrospun into consistent fibres with an average diameter of 0.9 μm (± 0.1 μm). Four and five percent PVB solutions created beaded fibres. A 8:2 ethanol:water solution lead to microbead formation while a 7:3 ethanol:water mix failed to fully dissolve. The selected solution was successfully electrospun onto a monolayer of human dermal fibroblasts and the process had no significant effect (p < 0.05) on cell viability compared to the control without fibres.
Conclusions:
PVB-ethanol-water solutions could be electrospun without damaging the exposed cell layer. However, further work is required to demonstrate the long-term effect of PVB as a wound healing material.
Related Concept Videos
Introduction to Fibroblasts
Directing Effect of Substituents: meta-Directing Groups
Directing Effect of Substituents: ortho–para-Directing Groups
[3,3] Sigmatropic Rearrangement of Allyl Vinyl Ethers: Claisen Rearrangement
Mnemonic Devices
Acronyms
Acronyms are created by using the initial letters of a series of words to form a new word or phrase. This approach condenses complex information into a single, memorable entity. For example,...
Non-ohmic Devices
Consider a simple circuit consisting of a battery, a diode, and a resistor. A...

